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In four saliva samples, we were unable to measure melatonin levels. The melatonin assay was performed after the cortisol assay, and in the case of two samples, there was not enough saliva for the melatonin assay. The other two cases were for saliva collected at , and one plausible reason might be that melatonin offset could have occurred earlier.

All P values and confidence limits were based on two-tailed calculations. All data generated or analysed during this study are included in this published article and its Supplementary Information files. Berson, D. Phototransduction by retinal ganglion cells that set the circadian clock. Science , — Hattar, S. Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity. Cajochen, C. High sensitivity of human melatonin, alertness, thermoregulation, and heart rate to short wavelength light. Wright, H. Effect of light wavelength on suppression and phase delay of the melatonin rhythm.

Gabel, V. Effects of artificial dawn and morning blue light on daytime cognitive performance, well-being, cortisol and melatonin levels. Figueiro, M. The effects of red and blue lights on circadian variations in cortisol, alpha amylase, and melatonin. Short-wavelength light enhances cortisol awakening response in sleep-restricted adolescents.

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Brain responses to violet, blue, and green monochromatic light exposures in humans: prominent role of blue light and the brainstem. PLoS One 2 , e Spectral quality of light modulates emotional brain responses in humans. Brainard, G. Action spectrum for melatonin regulation in humans: evidence for a novel circadian photoreceptor. Thapan, K. Miranda, J. From the internet of things to the internet of people. Mott, M. The supporting effects of high luminous conditions on grade 3 oral reading fluency scores.

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Springer Plus 3 , 53 Barkmann, C. Applicability and efficacy of variable light in schools.

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Choi, K. Dynamic lighting system for the learning environment: performance of elementary students. Express 24 , A—A Sleegers, P. Meek, C.

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Circadian effectiveness of two polychromatic lights in suppressing human nocturnal melatonin. Kozaki, T. Effects of short wavelength control in polychromatic light sources on nocturnal melatonin secretion. Rea, M. Human melatonin suppression by light: a case for scotopic efficiency. Chellappa, S.

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Non-visual effects of light on melatonin, alertness and cognitive performance: can blue-enriched light keep us alert? PLoS One 6 , e Differential impact in young and older individuals of blue-enriched white light on circadian physiology and alertness during sustained wakefulness. Jung, C. Acute effects of bright light exposure on cortisol levels. Rhythms 25 , — Time-of-day-dependent effects of bright light exposure on human psychophysiology: comparison of daytime and nighttime exposure. Sato, M. The effects of exposure in the morning to light of different color temperatures on the behavior of core temperature and melatonin secretion in humans.

Rhythm Res. Lack of short-wavelength light during the school day delays dim light melatonin onset DLMO in middle school students. Neuro Endocrinol. Light-induced melatonin suppression at night after exposure to different wavelength composition of morning light. Blue-enriched morning light as a countermeasure to light at the wrong time: effects on cognition, sleepiness, sleep, and circadian phase. Neuropsychobiology 74 , — Ahn, B.

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Individual variations in serum melatonin levels through time: implications for epidemiologic studies. PLoS one 8 , e Alerting effects of light. Sleep Med. Steffy, G. Architectural Lighting Design. DiLaura, D. The Lighting Handbook: Reference and Application.

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Lucas, R. Measuring and using light in the melanopsin age. Trends Neurosci. Maierova, L. Diurnal variations of hormonal secretion, alertness and cognition in extreme chronotypes under different lighting conditions. Gordon, G. Interior Lighting for Designers. Buysse, D. The Pittsburgh Sleep Quality Index: a new instrument for psychiatric practice and research. Psychiatry Res. Horne, J. A self-assessment questionnaire to determine morningness-eveningness in human circadian rhythms.

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Addicott, M. The effect of daily caffeine use on cerebral blood flow: How much caffeine can we tolerate? Brain Mapp. Effects of saliva collection using cotton swabs on melatonin enzyme immunoassay.

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Circadian Rhythms 9 , 1—4 Deacon, S. Posture influences melatonin concentrations in plasma and saliva in humans. Subjective and objective sleepiness in the active individual.